1. Check the autoclave's drain screen.
2. Prepare research materials for sterilization.
3. Use secondary containment for all items being autoclaved.
4. Close the autoclave door securely.
5. Choose the correct cycle.
6. Set the appropriate time for sterilization and drying.
7. Start the autoclave.
8. Fill out the autoclave log.
9. Follow these precautions when the autoclave cycle is finished.